Catalog-specification |
Delivery time |
USD price |
abs110765-50ul |
In Stock |
228 |
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Overview | |
Description |
A Rabbit Polyclonal Antibody (Pab) that has been purified through peptide affinity is available. To create new content related to the original text, the information can be rearranged. |
Other names |
Paired box protein Pax-8, PAX8ï¼›PAX8 |
Source |
Rabbit |
Specificity |
The production of this PAX8 antibody involved immunizing rabbits with a synthetic peptide, which was conjugated to KLH. The peptide spanned amino acids 285-313 from the Central region of the human PAX8 protein. To create a similar content, the above information can be rearranged as follows: |
Species Reactivity |
Human |
Predictive reaction species |
M |
Application |
WB~~1:1000 |
Properties | |
Clonality |
Polyclonal Antibody |
Isotype |
Rabbit Ig |
Stability & Storage |
To ensure optimal preservation, please keep the refrigerated temperature between 2-8°C for a maximum duration of 2 weeks. For extended storage, it is advisable to store the items at -20°C in small portions to prevent the negative effects of freeze-thaw cycles. |
Storage buffer |
This antibody is purified through peptide affinity purification followed by a protein A column to obtain a purified polyclonal antibody. It is supplied in PBS solution containing 0.09% (W/V) sodium azide as a preservative. |
Research area |
Neuroscience |
Target | |
Background |
One important regulator responsible for maintaining the unique gene expression profile in thyroid cells and preserving their functional identity is the transcription factor. It plays a crucial role in ensuring that only thyroid-specific genes are exclusively expressed in these cells, thereby supporting their specialized function. By orchestrating the intricate process of gene regulation, this transcription factor contributes significantly to the differentiation and proper functioning of thyroid cells. |
Celluar localization |
Nucleus. |
UniPort |
Q06710 |
References | |
References |
Ramos,H.E., et.al., Thyroid 19 (1), 61-68 (2009) |
Data Examples
All lanes: Anti-PAX8 Antibody (Center) at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of PAX8 Antibody (Center) (Cat. #abs110765) in HL-60 cell line lysates (35ug/lane). PAX8 (arrow) was detected using the purified Pab.
(LEFT)Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of 293 cells using PAX8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
(LEFT)Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of 293 cells using PAX8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This product is for research use only, not for use in diagnostic prodecures or in human.
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